KAPA Dual-Indexed Adapter Kits are compatible with all KAPA DNA and RNA library preparation kits, and provide an extended range of applications (compared to Single-Index adapters); including human and microbial whole-genome sequencing, as well as higher throughput targeted sequencing and RNA-Seq. KAPA Dual-Indexed Adapter Kits comprise 96 high-quality, ready-to-use adapters for Illumina® library construction, that include commonly used barcodes for seamless integration into multiplexed sequencing workflows together with Adapter Dilution Buffer.
KAPA Dual-Indexed Adapter Kits for Illumina platforms are designed for use with KAPA DNA and RNA library preparation kits to construct libraries for sequencing on an Illumina sequencer. Each plate contains a set of 96 adapters, each with two, 8-nucleotide indexes (barcodes) for multiplexed sequencing applications. The sequences of the sequencing indices (barcodes) included in KAPA Dual-Indexed Adapter Kits are given in Important Parameters: Index Sequences and Pooling Guidelines (p. 6). The highly dissimilar nature of indices minimizes the possibility of inaccurate index calling. KAPA Adapter Dilution Buffer [10 mM Tris-HCl, (pH 8.0 – 8.5), 10 mM NaCl, 1 mM EDTA] is provided with the kit to ensure optimal performance when adapters require further dilution.