The KAPA Frag Kit for Enzymatic Fragmentation provides for robust and reproducible enzymatic fragmentation of double-stranded DNA (dsDNA) across a wide range of sample types and inputs (1 ng – 1 μg), and may be incorporated in any NGS library construction work ow that requires fragmented dsDNA as the input.
The work ow is automation-friendly, and unlike mechanical shearing, does not require any specialized equipment or consumables. The degree of fragmentation (mode size and size distribution of DNA fragments) is controlled by fragmentation time and temperature. Optimal fragmentation parameters are somewhat dependent on the amount and nature of input DNA, but the KAPA Frag system is much less sensitive to DNA input and quality, and signi cantly more reproducible than other enzymatic fragmentation technologies, including tagmentation.
Libraries produced from DNA fragmented with the KAPA Frag system are functionally equivalent to libraries prepared from Covaris-sheared DNA.