KAPA High Throughput Library Preparation Kit

Material Number: N/A

KAPA HTP Library Prep. contains optimally formulated enzymes selected through our directed evolution technology and implements a highly optimized “with bead” strategy that enables higher library construction efficiency and facilitates automation.

KAPA HTP (High Throughput) Library Preparation Kit

KAPA High Throughput (HTP) LTP Library Preparation Kits contain optimally formulated enzymes selected through our directed evolution technology and implement a highly optimized “with bead” strategy that enables higher library construction efficiency and facilitates automation.

Features and Benefits of KAPA HTP/LTP Library Preparation Kits

  • Automation-friendly, highly optimized “with-bead” protocol facilitating minimal sample loss with no physical transfer of library material between enzymatic reactions
  • Improved coverage uniformity as a result of low-bias library amplification with KAPA HiFi enzyme
  • Compatibility with a wide range of DNA sample types, inputs and sequencing applications, and flexibility with respect to fragment size, adapter design, and library amplification
  • Qualified automation methods

KAPA HTP Library Preparation Kit is designed for high-throughput library construction for Illumina sequencing, starting from fragmented, double-stranded DNA (dsDNA). The kit contains

  • End Repair Buffer (10X)
  • End Repair Enzyme Mix
  • A-Tailing Buffer (10X)
  • A-Tailing Enzyme
  • Ligation Buffer (5X)
  • DNA Ligase
  • PEG/NaCl Solution
  • KAPA HiFi HotStart ReadyMix (2X)
  • Library Amplification Primer Mix (10X)

Applications

KAPA HTP Library Preparation Kits are ideally suited for high-throughput NGS library construction workflows that require end repair, A-tailing, adapter ligation and library amplification (optional). The kit is designed for library construction from a wide range of sample types and inputs(10 ng – 5 µg). The protocol is automation friendly and may be incorporated into workflows for a wide range of NGS applications, including:

  • whole-genome, shotgun sequencing
  • whole-exome or targeted sequencing, using Roche SeqCap EZ, Agilent SureSelect, Illumina TruSeq, IDT xGen Lockdown Probes, or other hybridization capture systems
  • ChIP-seq
  • RNA-seq (starting with cDNA)
  • methyl-seq (in combination with the KAPA HiFi HotStart Uracil+ ReadyMix for library amplification).

KAPA High Throughput Library Preparation Kit References are:

KAPA High Throughput Library Preparation Kit – 96 rxn | 7961901001 – KK8234
KAPA High Throughput Library Preparation Kit – 96 rxn PCR-free | 7961910001 – KK8235

 

Discover here our other HiFi offering.

For more information, please visit our Technical portal.