KAPA HTP (High Throughput) Library Preparation Kit
KAPA High Throughput (HTP) LTP Library Preparation Kits contain optimally formulated enzymes selected through our directed evolution technology and implement a highly optimized “with bead” strategy that enables higher library construction efficiency and facilitates automation.
Features and Benefits of KAPA HTP/LTP Library Preparation Kits
- Automation-friendly, highly optimized “with-bead” protocol facilitating minimal sample loss with no physical transfer of library material between enzymatic reactions
- Improved coverage uniformity as a result of low-bias library amplification with KAPA HiFi enzyme
- Compatibility with a wide range of DNA sample types, inputs and sequencing applications, and flexibility with respect to fragment size, adapter design, and library amplification
- Qualified automation methods
KAPA HTP Library Preparation Kit is designed for high-throughput library construction for Illumina sequencing, starting from fragmented, double-stranded DNA (dsDNA). The kit contains
- End Repair Buffer (10X)
- End Repair Enzyme Mix
- A-Tailing Buffer (10X)
- A-Tailing Enzyme
- Ligation Buffer (5X)
- DNA Ligase
- PEG/NaCl Solution
- KAPA HiFi HotStart ReadyMix (2X)
- Library Amplification Primer Mix (10X)
Applications
KAPA HTP Library Preparation Kits are ideally suited for high-throughput NGS library construction workflows that require end repair, A-tailing, adapter ligation and library amplification (optional). The kit is designed for library construction from a wide range of sample types and inputs(10 ng – 5 µg). The protocol is automation friendly and may be incorporated into workflows for a wide range of NGS applications, including:
- whole-genome, shotgun sequencing
- whole-exome or targeted sequencing, using Roche SeqCap EZ, Agilent SureSelect, Illumina TruSeq, IDT xGen Lockdown Probes, or other hybridization capture systems
- ChIP-seq
- RNA-seq (starting with cDNA)
- methyl-seq (in combination with the KAPA HiFi HotStart Uracil+ ReadyMix for library amplification).
KAPA High Throughput Library Preparation Kit References are:
KAPA High Throughput Library Preparation Kit – 96 rxn | 7961901001 – KK8234
KAPA High Throughput Library Preparation Kit – 96 rxn PCR-free | 7961910001 – KK8235
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