The KAPA Stranded RNA-Seq Kit offers a high-quality, comprehensive solution for transcriptome sequencing. The workflow is designed for both manual and automated NGS library construction from 10 – 400 ng of total, rRNA-depleted, or poly(A)-enriched RNA.
The protocol is applicable to a wide range of RNA-seq applications, including targeted RNA-seq, whole transcriptome, gene expression analysis of high- and low-quality RNA samples (e.g., extracted from FFPE tissue) for single nucleotide variation (SNV) discovery and splice junction and gene fusion identification.
In order to maximize sequence coverage uniformity and to maintain relative transcript abundance, it is critical that library amplification bias be kept to a minimum. KAPA HiFi DNA Polymerase has been designed for low-bias, high fidelity PCR, and is the polymerase of choice for NGS library amplification.