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LightCycler® 480 System Technology
General Design The LightCycler® 480 Real-Time PCR System features a compact and versatile benchtop instrument that includes interchangeable silver thermal block cycler unit. Users can flexibly switch between 96- and 384-mulitiwell plate formats, or choose between clear or white LightCycler® 480 multiwell plates. Figure 1: LightCycler® 480 Instrument with workstation Figure 2: Inner view of the LightCycler® 480 […]
The top advantages and disadvantages of different RNA extraction methods
Isolating high-quality RNA is the most critical step for successfully performing a broad range of assays, from RT-qPCR or microarray analysis to cDNA library preparation, as well as Northern blot studies. It is even critical for high-throughput transcriptome analysis using next-generation sequencing techniques. Therefore, getting the most from your RNA isolation procedure is a must. […]
LightCycler® 480 Detection Formats
Due to its highly flexible selection of excitation and emission wavelengths, the LightCycler® 480 Real-Time PCR System is compatible with most currently available dyes and assay formats, yet is also adaptable to new, future applications. The following assay formats can be used on the LightCycler® 480 Real-Time PCR System: SYBR Green I HybProbe Probes Hydrolysis Probes SimpleProbe […]