KAPA HiFi HotStart Library Amp Kit

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KAPA HiFi Hotstart Library Amp Kit has been designed to address PCR-induced bias.

KAPA HiFi HotStart Library Amp Kit

KAPA Library Hotstart Library Amp Kit has been designed to address PCR-induced bias. The Kit contains the novel KAPA HiFi DNA Polymerase, engineered for high-fidelity and processivity and capable of balanced amplification of complex library DNA. The Kit is supplied as a ready-to-use master mix (2X) containing all components for PCR, except primers and template.

KAPA Library Amplification Kits for Illumina platforms are designed for the amplification of next-generation sequencing libraries prepared for Illumina sequencing.

In order to maximize sequence coverage uniformity, it is critical to minimize library amplification bias. KAPA HiFi DNA Polymerase is designed for low-bias, high-fidelity PCR, and is the reagent of choice for NGS library amplification.1,2,3,4.

KAPA Library Amplification Kits include

  • KAPA HiFi Hotstart DNA Polymersa (1 U/µL),
  • Fidelity Buffer (5X),
  • GC Buffer (5x),
  • High-quality dNTPs (10 mM each),
  • MgCl2 (25 mM)

KAPA HiFi HotStart is also available in KAPA HiFi HotStart ReadyMix , which contains DNA Polymerase, reaction buffer, dNTPs and MgCl2 (at a final concentration of 2.5 mM).

Learn more about product specifications here.

1. Oyola, S.O., et al., BMC Genomics 13, 1 (2012).
2. Quail, M.A., et al., Nature Methods 9, 10 (2012).
3. Quail, M.A., et al., BMC Genomics 13, 341 (2012).
4. Ross, M.G., et al., Genome Biology 14, R51 (2013).


Product variations

Material Number Name Price
07958960001 KAPA HiFi HotStart Library Amp Kit - 250 rxn Log in or register to see the price