KAPA HyperPETE Panels, Refine precision
RNA gene fusion detection and more..
The latest addition to the KAPA portfolio is now available. KAPA HyperPETE Workflow, enhancing your target enrichment workflow, for DNA and RNA, specifically designed for panels 250KB and under. Learn more about the newest Panel NGS Workflow by Roche here.
Features & Benefits
- Better by design, using Roche’s renowned content and panel design expertise.
- High uniformity for better sequencing efficiency with less optimization.
- Readily available for fast delivery.
- Validated NAVIFY® Mutation Caller secondary analysis solution available.
Contact us and learn about the new Target enrichment revolution.
KAPA HyperPETE, Refine Precision.
The most frequently used enrichment methods for next-generation sequencing (NGS) are hybridization or capture-based target enrichment and amplicon-based target enrichment. While the capture-based method uses specific probes for enrichment, amplicon-based methods use the power of PCR to do the same.
There are advantages and disadvantages to both methods; choosing the right method may depend on the type of application where it is used. A new innovative method based on Primer Extension Target Enrichment (PETE) aims to combine the advantages of capture-based and amplicon-based target enrichment.
Taking into account all these variations, it is important to use library preparation reagents that can work efficiently with all these sample types. Depending on the end goal (for example, whole exome sequencing), a target enrichment step may also be required for enriching specific regions of interest
In our spirit of innovation, Roche continues to raise the bar for target enrichment workflows and gene panel sequencing
To drive more efficient and accurate variant identification, KAPA HyperPETE panels are supported by an integrated secondary analysis solution, NAVIFY® Mutation Caller, which offers pipelines for somatic SNV, Indel and CNV calling, microsatellite instability scoring, both known and unkown RNA fusion detection, and germline SNV and Indel calling. Combining the precision of the KAPA HyperPETE Workflow for small panels and the validation capability of NAVIFY® Mutation Caller, you can be more confident in every result.
Future-proof your lab with regular software updates and algorithm maintenance from Roche, giving you the support you need to free up resources and create a sustainable operation. Use the comprehensive design tool HyperDesign to design your own custom panels and take advantage of the Roche’s expert designers help.
Strong and reliable variant call performance
The accuracy and precision of the KAPA HyperPETE Workflow enhances performance throughout the sample journey, with:
- Precise targeting of difficult genomic regions increases confidence in variant detection
- Higher target coverage offers robust support for varying sample types and variants
- Single-day high performing workflow
- A lower duplicate rate helps mitigate error and improve accuracy
- Design your KAPA HyperPETE personalized panels with HyperDesign
*The missed fusion was EGFR-SEPT14 due to sequence similarity of SEPT14 with another non-coding RNA transcript. RNA fusion caller randomly picked different transcripts with similar sequences for alignment.
The KAPA HyperPETE Pan Cancer Panel is a 302 Kb capture target panel covering 1324 target regions, 86 cancer related genes and 190 MSI loci relevant to somatic oncology research applications.The panel’s been optimized to deliver high uniformity and specificity from low quality, low quantity FFPET DNA and cfDNA samples.
The KAPA HyperPETE Hot Spot Panel is a 40 Kb capture target panel covering 37 targets from the genomic ‘hot spot’ regions that are frequently mutated in human cancer genes. It is optimized to deliver high uniformity and specificity from low quality, low quantity FFPET DNA and cfDNA samples.
The KAPA HyperPETE Hereditary Oncology Panel is a 200 Kb capture target panel focusing on hereditary breast and ovarian cancer research (including BRCA1 and BRCA2) as well as hereditary colorectal cancer research. This Oncology panel covers 47 related genes in these cancer types and has been optimized to deliver high uniformity and specificity in germline variant research with the pre-capture multiplexing capacity of 8 samples.
The KAPA HyperPETE Lung Cancer Fusion Panel is a 18 Kb capture target panel also using the novel KAPA HyperPETE technology to detect the known fusions and unknown fusion partners including 17 lung cancer fusion genes and 4 housekeeping genes (as internal controls).
The KAPA HyperPETE Newborn Screening Panel is a 290 Kb capture target panel covering 89 related genes. It is optimized to deliver high uniformity and specificity in germline variant research with the pre-capture multiplexing capacity of 8 samples.
YOUR GUIDE TO SUCCESS
Uncover all the features and benefits. Download here the brochure.
Research Use Only. Not for use in diagnostic procedures.
HYPERDESIGN, NAVIFY, KAPA HYPERPREP, KAPA HYPERPETE, HIGH PURE and KAPA are trademarks of Roche. All other product names and trademarks are the property of their respective owner.