Simultaneous Gene Expression Analysis and Fusion Identification by Pairing the KAPA RNA EvoPrep Kit with KAPA HyperExome V2 Probes
While widely adopted for comprehensive transcriptome profiling, the methods of poly-A selected or ribodepleted RNA-sequencing may have key limitations, including input mass requirements, inefficient fusion detection, and wasted reads on non-coding transcripts. The detection of fusion transcripts—which are present at low but potentially biologically relevant levels in many tumor types—requires enrichment techniques. However, many on-market fusion panels are unable to profile the entire transcriptome, leading to incomplete gene expression data and limiting insights.
To overcome these challenges, Roche demonstrates a new workflow using the new KAPA RNA EvoPrep Kit paired with KAPA HyperExome V2 Probes, which can successfully be used for enrichment of both DNA and RNA libraries. The workflow enables enrichment of protein-coding genes to be used simultaneously in gene expression analysis and detection of fusion and exon-skipping events. The speaker will demonstrate key advancements in RNA library preparation using both high-quality and formalin fixed paraffin embedded (FFPE) samples.
Attendees can learn about:
- High exonic mapping of reads—and fewer wasted reads—through the flexible design of KAPA HyperExome V2 Probes.
- High concordance of results between RNA inputs of variable qualities and mass.
- Biologically relevant gene expression, with few genes significantly differentially expressed between enriched data and standard whole-transcriptome methods.
- High detection rate for fusions and exon-skipping events starting with as little as 10 ng of input RNA (downsampled to 30 million paired-end reads).
For Research Use Only. Not for use in diagnostic procedures.
Webinar speakers
Amy Elias
PhD Applications Scientist Sequencing & Applications Roche Diagnostics
Amy Elias has nine years of hands-on and bioinformatic RNA experience. Currently, she is an applications scientist within Roche Sequencing and Life Science. She proudly develops applications tailored towards customer and market needs and helps assist with customer workflows. Amy Elias earned her PhD in Molecular Biology, Cell Biology, and Biochemistry, with a focus on the Biology of Aging, from Brown University in Providence, Rhode Island, USA in 2022.
Shandré Janse van Vuuren
International Product Manager
Sample Prep Reagents, Sequencing Systems
Roche Diagnostics
Shandré Janse van Vuuren currently serves as the International Product Manager for Sample Prep Reagents in Sequencing at Roche Diagnostics. In this pivotal role, she directs the comprehensive KAPA RNA Library Prep Portfolio, fostering its innovation and influencing the trajectory of new product development and refinement. Shandré possesses a Master of Science degree in Biochemistry from the esteemed University of Stellenbosch, South Africa.
Ray Sodha
International Product Manager
Sample Prep Reagents, Sequencing Systems
Roche Diagnostics
Ray Sodha serves as International Product Manager, supporting the Target Enrichment portfolio in the Sample Prep Reagents team in Sequencing at Roche Diagnostics. He directs the KAPA HyperCap portfolio including a wide range of custom products, as well as catalog panels including the KAPA HyperExome v2 Probes. He brings over fifteen years of experience in commercializing various technologies in biomedical applications with a Master of Science in biology and technology commercialization from Case Western University, Cleveland, USA.
