High Pure Viral Nucleic Acid Kit
The High Pure Viral Nucleic Acid Kit provides a streamlined solution for the purification of viral nucleic acids from mammalian serum, plasma, or whole blood, readying samples for direct analysis via PCR or RT-PCR in PCR-grade water. This comprehensive kit facilitates the isolation of both viral RNA and DNA from whole blood, integrating specialized components such as an Inhibitor Removal Buffer and glass fibers to enhance assay accuracy. Ideal for a range of viral detection tasks, it ensures high-quality nucleic acid preparation for reliable RT-PCR outcomes.
Benefits of the High Pure Viral Nucleic Acid Kit
- Isolates both viral RNA and DNA, allowing simultaneous detection of both types of virus
- Removes inhibitors that might interfere with downstream assays, ensuring greater assay specificity, sensitivity and reproducibility
- Prepares nucleic acid samples in only 20 minutes
- Yields a concentrated sample that is suitable for direct assay (no precipitation required)
- Procedure is scalable, to accommodate samples with low viral loads
How this product works
As a pre-requisite for the analysis of viral nucleic acids by PCR or RT-PCR, the isolation of the analyte from serum, plasma, or whole blood is required.
Virus lysis is accomplished by incubation of the sample in a special Lysis/Binding Buffer in the presence of Proteinase K. Subsequently, nucleic acids bind specifically to the surface of glass fibers in the presence of a chaotropic salt. The binding reaction occurs within seconds due to the disruption of the organized structure of water molecules and the interaction of nucleic acids with the glass fibers surface. Thus, adsorption to the glass fiber fleece is favored. Since the binding process is specific for nucleic acids, the bound nucleic acids are purified from salts, proteins, and other impurities by a washing step and are eluted in low-salt buffer or water.
- Serum, plasma, or whole blood are lysed by incubation with Binding Buffer and Proteinase K.
- Nucleic acids are bound to the glass fibers pre-packed in the High Pure Filter Tube.
- Bound nucleic acids are washed with a special Inhibitor Removal Buffer to get rid of PCR inhibitory contaminants.
– Allows even the application of heparinized sample material with >100 U/mL heparin. - Washing of bound nucleic acids, purification from salts, proteins, and other cellular impurities.
- Purified nucleic acids are recovered using the Elution Buffer.
Figure: Crossing Points of a series dilution of HBV and HCV particles in human Citrate Plasma after isolation with the High Pure Viral Nucleic Acid Kit and subsequent analysis on the LightCycler ® 480 Instrument II
