KAPA HiFi HotStart Uracil+ ReadyMix
It is a modified version of KAPA HiFi HotStart, engineered to tolerate uracil residues. KAPA HiFi HotStart provides high fidelity NGS library amplification with the highest yield, lowest bias, and most uniform sequence coverage available.
KAPA HiFi HotStart is a novel B-family DNA polymerase, engineered to have increased affinity for DNA, without the need for accessory proteins or DNA-binding domains. The intrinsic high processivity of the enzyme results in significant improvement in yield, speed and sensitivity when compared with wild-type B-family DNA polymerases and polymerase blends. When used for the amplification of next-generation sequencing (NGS) libraries, KAPA HiFi HotStart DNA Polymerase exhibits high yields with minimal amplification bias and provides extremely uniform sequence coverage.
KAPA HiFi HotStart Uracil+ ReadyMix contains all components for PCR, except primers and template.
KAPA HiFi Hotstart Uracil+ Benefits
- Higher and more uniform bisulfite-sequencing coverage for templates containing uracil compared to other methods
- Higher yields, low duplication rates and fewer wasted sequencing reads
- Convenience with the consistency of one core enzyme
- Trusted as shown through over 10 years in the industry as well as thousands of peer-reviewed publications
The read-ahead function of proofreading DNA polymerases detects pro-mutagenic uracil residues in the template strand and prevents further strand extension, thereby reducing or completely inhibiting PCR amplification. In KAPA HiFi HotStart Uracil+ DNA Polymerase, this uracilbinding pocket is inactivated to enable the amplification of uracil-containing DNA. The enzyme shows the same high yield, low GC-bias and coverage uniformity as the unmodified KAPA HiFi HotStart DNA Polymerase, making it particularly advantageous for applications employing bisulfite DNA conversion, which typically produces low concentrations of AT-rich DNA.
The enzyme has 5’-3’ polymerase and 3’-5’ exonuclease (proofreading) activity, but no 5’g3’ exonuclease activity. The strong 3’-5’ exonuclease activity results in superior accuracy during DNA amplification. A proprietary antibody inactivates the polymerase until the first cycle of denaturation, minimizing spurious amplification products that may result from nonspecific priming events during reaction setup and initiation, and increasing overall reaction efficiency.
The ReadyMix contains 0.2 mM of each dNTP (dATP, dCTP, dGTP, dTTP), and does not contain dUTP.
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Find more information about Library Amplification here
The name of this product was recently changed. The old name was: KAPA High Fidelity HotStart Uracil with ReadyMix