|   Application Notes, Sequencing

Sequencing workflows are ever changing: on-going research and technological innovation allow for the development of new tools and options to improve and optimise existing protocols. So, what do you know about the latest solutions? To help you get all the information you need, we selected seven KAPA application notes on the benefits, application and results of KAPA Kits.

Find out which topics the application notes cover in the overview below.

Application note 1:

A qualified, streamlined, RNA-Seq solution for a gene expression analysis

RNA-Seq: Gene Expression Analysis

RNASeq is a powerful tool for gene expression, which requires dedicated and strong qualified data analysis pipelines. KAPA RNA HyperPrep Kits and the Genialis platform offer simple and complete workflow solutions for NGS-based gene expression analysis, leaving you more time to focus on biological questions. Learn more about this solution and its benefits in this first application note.


Application note 2:

A flexible technology for selective transcript depletion prior to library construction

Selective transcript depletion

KAPA RiboErase (HMR) Kits support the efficient depletion of and mitochondrial rRNA and globin transcripts from human, mouse and rat samples in a 2,5 hour workflow. This application note describes the highly flexible and automation-friendly protocol, and discusses how it can easily be adapted for user-supplied oligo’s to deplete other transcripts.


Application note 3:

High-efficiency species-specific ribosomal RNA depletion with the KAPA RNA HyperPrep Kit

Species-specific ribosomal RNA depletion

Enzymatic ribosomal RNA (rRNA) depletion is the first step to maximise coverage of target RNA to improve and optimise sequencing economy for human, mouse and rat species. This is why KAPA RNA HyperPrep’s workflow includes RiboErase. This application note describes the results of applying uniquely custom designed oligo’s to enable specific depletion of E. coli rRNA.


Application note 4:

A reliable, single-day library prep solution for whole transcriptome sequencing from FFPE-derived RNA

Whole Transcriptome Sequencing from FFPE-derived RNA

FFPE derived RNA can be highly variable due to the damaging nature of the fixation process (which may result in crosslinking, chemical modification, and fragmentation), the age of the tissue, and the RNA extraction method used. KAPA RNA HyperPrep Kits support reliable transcriptome sequencing results from degraded inputs. Different library prep strategies can be followed by enrichment for mRNA (capture), or depletion of rRNA with RiboErase (human, mouse, rat). Learn more about these strategies in this application note.


Application note 5:

KAPA HyperPrep Kits offer a flexible, high-efficiency library preparation solution for PCR-free human whole-genome sequencing

Human whole-genome sequencing

Routine human whole-genome sequencing (WGS) requires robust and streamlined PCR-free library preparation protocols that can be tailored to ensure optimal sequencing results on production-scale Illumina® sequencers.

This application note discusses how KAPA provides a complete sample prep workflow solution for efficient use of Illumina’s HiSeq® and NovaSeq® instruments, by combining all necessary components: KAPA HyperPrep kit, KAPA DI-Adapters, KAPA Pure Beads, and KAPA Library Quantification Kit.


Application note 6:

Fast turnaround times and improved data quality for microbial whole-genome sequencing

Microbial whole-genome sequencing

Next-generation whole genome sequencing of microbes demands rapid, robust, and scalable library construction workflows, capable of generating high-quality sequence data across a wide range of genome sizes, complexities and genomic GC content. In this application note, we describe a novel, single-tube enzymatic fragmentation and library construction method that results in minimal bias, high uniform coverage, and facilitates de novo assembly of microbial genomes.


Application note 7:

A streamlined bacterial whole-genome sequencing workflow, utilizing crude lysates from single colonies

Crude sample bacterial whole-genome sequencing

Whole Genome sequencing of bacterial isolates requires many time consuming and laborious steps. The KAPA HyperPlus Kit workflow overcomes bacterial culturing and gDNA isolation, by starting the preparation of gDNA libraries directly from single-colony crude cell lysates. Sequencing data confirms comparable results when starting with purified bacterial gDNA. Learn more about the protocol and the results in this application note.