KAPA HyperPlus Kit

Material Number: Select a variation

The KAPA HyperPlus kit provides a streamlined workflow that includes fragmentation and library preparation in a single tube.

KAPA HyperPlus Kit

The KAPA HyperPlus kit provides a streamlined workflow that includes fragmentation and library preparation in a single tube. Building on industry-leading library construction efficiencies, this integrated solution combines enzymatic fragmentation, similar in quality to mechanical shearing, with the speed and convenience of tagmentation-based workflows.

Features and Benefits of KAPA HyperPlus Kits

Fully automatable DNA library construction in 1.5-3 hours with low-bias enzymatic fragmentation
Industry-leading conversion rates and library complexity,* particularly for FFPE DNA
Now includes an additional End Repair and A-Tailing module for more sensitive applications with no change to the workflow
PCR-free workflows from lower inputs and improved sequence coverage in workflows with PCR
Compatibility with a wide range of sample types and inputs, and flexibility with respect to fragment size, adapter design, and library amplification

KAPA HyperPlus Kits are ideally suited for low- and high-throughput NGS library construction workflows that require DNA fragmentation, end-repair, A-tailing, adapter ligation, and library amplification (optional). Kits are designed for library construction from a wide range of sample types and inputs (1 ng – 1 μg), and are compatible with complex, genomic DNA; low-complexity samples such as small viral genomes, plasmids, cDNA and long amplicons; and low-quality DNA such as FFPE samples.

The entire workflow is automation-friendly and may be incorporated into workflows for a wide range of NGS applications, including:

whole-genome, shotgun sequencing.
whole-exome or targeted sequencing, using KAPA HyperCap Target Enrichment Kits, Agilent SureSelect, Illumina TruSeq, IDT xGen Lockdown Probes, or other hybridization capture systems.
RNA-seq (starting with cDNA).