KAPA Pure Beads is a suspension of paramagnetic beads in a buffer optimized for the purification or size selection of single- and double-stranded DNA in next-generation sequencing and other molecular biology workflows. The product is designed for fast and reliable purification or size selection of 1 ng to 5 µg DNA in a single reaction. KAPA Pure Beads is compatible with manual processing or automated liquid handling and enables efficient recovery of input DNA in both formats. For purification and size selection of DNA, the KAPA Pure Beads buffer includes PEG/NaCl—a crowding agent designed to drive DNA molecules to the beads for binding. The volumetric ratio of KAPA Pure Beads to sample is the critical factor in determining the size distribution of DNA fragments retained by the beads. The volume (ratio) may be modified/optimized based upon the specific application and/or point in the library construction workflow where a cleanup or size selection is employed.
KAPA Pure Beads is ideally suited for:
- fast and efficient reaction cleanups to remove adapters, adapter-dimers, primers, primer-dimers, nucleotides, salts, and enzymes in NGS library preparation, PCR, and qPCR workflows,
- size selection of fragmented input DNA, adapterligated library molecules, or amplified libraries in NGS library preparation workflows, and
- general manipulation of DNA samples, e.g., sample concentration and buffer exchange (e.g., from salts, buffer components, enzymes, etc.).