The KAPA mRNA EvoPrep Kit contains all of the buffers and enzymes required for poly(A) mRNA capture and the rapid construction of stranded mRNA-Seq libraries from 25 ng – 1000 ng of intact total RNA. The KAPA mRNA EvoPrep Kit is designed for both manual and automated NGS library construction. The kit enriches for polyadenylated RNAs using magnetic oligo-dT beads.
The protocol is applicable to a wide range of RNA-Seq applications, including:
- gene expression analysis;
- single nucleotide variation (SNV) discovery;
- splice junction and gene fusion identification; and
- characterization of polyadenylated RNAs
This kit has not been tested with small RNAs < 100 bp in length.
