KAPA Library Amplification Kits have been designed to address PCR-induced bias. Kits contain the novel KAPA HiFi DNA Polymerase, engineered for highfidelity and processivity and capable of balanced amplification of complex library DNA. Kits are supplied as a ready-to-use master mix (2X) containing all components for PCR, including primers. Template not included.
KAPA Library Amplification Kits for Illumina platforms are designed for the amplification of next-generation sequencing libraries prepared for Illumina sequencing.
In order to maximize sequence coverage uniformity, it is critical to minimize library amplification bias. KAPA HiFi DNA Polymerase is designed for low-bias, highfidelity PCR, and is the reagent of choice for NGS library amplification.1,2,3,4 KAPA Library Amplification Kits include KAPA HiFi HotStart ReadyMix (2X), a ready-touse PCR mix comprising all the components for library amplification— except primers and template. Kits also include Library Amplification Primer Mix (10X), designed for the high-efficiency amplification of Illumina libraries flanked by adapters containing the P5 and P7 flow cell sequences.
1. Oyola, S.O., et al., BMC Genomics 13, 1 (2012).
2. Quail, M.A., et al., Nature Methods 9, 10 (2012).
3. Quail, M.A., et al., BMC Genomics 13, 341 (2012).
4. Ross, M.G., et al., Genome Biology 14, R51 (2013).