KAPA Library Amplification Kit with Primer Mix

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KAPA Library Amplification Kits have been designed to address PCR-induced bias.

KAPA Library Amplification Kits have been designed to address PCR-induced bias. Kits contain the novel KAPA HiFi DNA Polymerase, engineered for highfidelity and processivity and capable of balanced amplification of complex library DNA. Kits are supplied as a ready-to-use master mix (2X) containing all components for PCR, including primers. Template not included.

KAPA Library Amplification Kits for Illumina platforms are designed for the amplification of next-generation sequencing libraries prepared for Illumina sequencing.

In order to maximize sequence coverage uniformity, it is critical to minimize library amplification bias. KAPA HiFi DNA Polymerase is designed for low-bias, highfidelity PCR, and is the reagent of choice for NGS library amplification.1,2,3,4 KAPA Library Amplification Kits include KAPA HiFi HotStart ReadyMix (2X), a ready-touse PCR mix comprising all the components for library amplification— except primers and template. Kits also include Library Amplification Primer Mix (10X), designed for the high-efficiency amplification of Illumina libraries flanked by adapters containing the P5 and P7 flow cell sequences.

1. Oyola, S.O., et al., BMC Genomics 13, 1 (2012).
2. Quail, M.A., et al., Nature Methods 9, 10 (2012).
3. Quail, M.A., et al., BMC Genomics 13, 341 (2012).
4. Ross, M.G., et al., Genome Biology 14, R51 (2013).


Product variations

Material Number Name Price
07958978001 KAPA Library Amplification Kit with Primer Mix - 50rxn Log in or register to see the price
07958986001 KAPA Library Amplification Kit with Primer Mix - 250rxn Log in or register to see the price