Uniquely Dual Indexed (UDI) primer pairs, each pair is uniquely barcoding each different sample during the pre-Capture Library amplification step.
The KAPA Unique Dual-Indexed (UDI) Primer Mixes are used in conjunction with KAPA Universal Adapters to generate uniquely labeled libraries from individual biological DNA samples. Sample indexing during library amplification allows for pooling of libraries prior to target capture or cluster generation, and enables multiplexed sequencing. Each KAPA UDI Primer Mix is a pre-mixed combination of forward and reverse primers. The primers contain a non-redundant (unique), 8-nucleotide index designed to mitigate index misalignment (“index hopping”) on Illumina sequencers that employ patterned flow cells and exclusion amplification chemistry.