LightMix® Digital EGFR_Ex19del
Using PCR methodology, an 87 bp fragment (wild-type sequence) covering the EGFR exon 19 deletions is amplified with specific primers. The mutation site is analyzed using two labeled probes: a reference probe (HEX-labeled) and an indel probe (FAM-labeled). The reference probe is designed to bind to the amplicon irrespective of mutation presence, and the indel probe is designed to bind to the wild-type sequence but not to any of the mutated sequences. The PC included in the kit contains 4-6 % synthetic mutant target in wild-type genomic DNA and is equivalent to ~17 ng/µl when dissolved in 60 µl of PCR grade water. It is recommended to always include at least one PC and a No- Template-Control (NTC) (e.g. PCR grade water) in each run.
