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LightMix® Digital KRAS G12D

Material Number: 10068531001
The LightMix® Digital KRAS G12D allows the detection of the G12D mutation in gene KRAS (COSMIC ID: COSM521) in genomic human DNA from a nucleic acid extract. It is a kit with reagents for a maximum of 32 (HiSense plate) or 46 (Universal plate) digital PCR reactions
Quantity: For a maximum of 32 (HiSense plate) or 46 (Universal plate) digital PCR reactions
Additional information

LightMix® Digital KRAS G12D

Using PCR methodology, a 72 bp fragment covering the KRAS G12D mutation is amplified with specific primers. The mutation site in the PCR fragment is analyzed using two labeled probes binding competitively to the mutation site. The mutant probe is FAM- labeled, and the wild type (WT) probe is HEX-labeled. The PC included in the kit contains 4-6 % synthetic mutant target in wild-type genomic DNA and is equivalent to ~17 ng/µl when dissolved in 60 µl of PCR grade water. It is recommended to always include at least one PC and a No-Template-Control (NTC) (e.g. PCR grade water) in each run.

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