Discover Our Comprehensive NGS Library Preparation Kits: KAPA EvoPrep & KAPA EvoPlus V2
Browse our portfolio of KAPA EvoPrep and KAPA EvoPlus V2 kits designed for efficient and high-quality next-generation sequencing library construction.
For Research Use Only. Not for use in diagnostic procedures.
Roche’s innovative KAPA EvoPrep Kits represent the cutting edge in NGS library preparation technology. Designed for maximum efficiency and compatibility with challenging sample types, these kits deliver superior performance while streamlining your workflow. Featuring the advanced KAPA EvoT4 DNA Ligase and automation-friendly formats, KAPA EvoPrep Kits help researchers achieve higher conversion rates and accelerate their path to breakthrough discoveries.
Introducing the KAPA EvoPlus V2 Kits, Roche’s advanced enzymatic fragmentation and library preparation solution. These upgraded kits deliver superior performance with improved fragmentation, better resistance to inhibitors, and higher conversion efficiency. Designed with automation in mind, the streamlined workflow reduces sequencing artifacts while increasing confidence in results. The KAPA EvoPlus V2 Kits represent the next evolution in NGS library preparation technology for researchers demanding maximum efficiency and reliability.
KAPA EvoPrep Kits
The KAPA EvoPrep Kits are the latest high-performance, streamlined, and automation-friendly library preparation solutions from Roche. The kits are validated with challenging sample inputs such as cell-free DNA, FFPET DNA, or mechanically sheared DNA, and developed to help reduce workflow steps. Their ReadyMix reagents are available in tube and plated formats for enhanced automatability and include the KAPA EvoT4 DNA Ligase (in the ligation ReadyMix). This new evolved ligase is specifically engineered to increase library conversion rates.
These and the many other advanced features of the KAPA EvoPrep Kits were thoughtfully designed to set new standards in NGS sample prep workflows and jumpstart the journey to your next big research breakthrough.
KAPA EvoPrep Kits offer a complete library preparation solution when combined with KAPA Adapters and KAPA HyperPure Beads (sold separately) and a complete target enrichment solution when combined with KAPA HyperCap or KAPA HyperPETE workflows. The kits are compatible with the Illumina sequencing platform and have been qualified with automation methods.
Benefits of the KAPA EvoPrep Kits*
* Compared to earlier KAPA DNA Libray Prep chemistries. Data on file with Roche.
- Exceptional library yields and sequencing quality
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Designed to provide increased library conversion efficiency with the KAPA EvoT4 DNA Ligase in the Ligation ReadyMix, enabling higher sensitivity and more confident variant detection
- Simplified and streamlined workflow
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Simplified, streamlined and automatable workflow with ReadyMix reagents, available in tube and plated format to increase efficiency and convenience
- Enable superior performance and sequencing efficiency
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Optimal utilization of sequencing throughput with minimal sequence coverage bias, minimization in the presence of sequencing artefacts with more unique library molecules recovered
Enhance your research with KAPA EvoPrep's higher conversion rates
Frequently asked questions about KAPA EvoPrep
- Whole-genome shotgun sequencing
- Whole-genome or targeted sequencing, using KAPA HyperCap Workflows or KAPA HyperPETE Workflows
- End repair and A-tailing, which produces end-repaired, 5′-phosphorylated, 3′-dA-tailed, dsDNA fragments
- Adapter ligation, during which dsDNA adapters with 3′-dTMP overhangs are ligated to 3′-dA-tailed library fragments
- Library amplification (optional), which employs high-fidelity, low-bias PCR to amplify library fragments carrying appropriate adapter sequences on both ends
The library construction process, from end repair and A-tailing to final library, can be performed in ~ 3 hours depending on experience, the number of samples being processed, and whether or not library amplification is performed. If necessary, the protocol may be paused safely after completion of the post-ligation cleanup or after post-amplification cleanup.
Purified, adapter-ligated library DNA may be stored at +2°C to +8°C for 1 – 2 weeks, or at -15°C to -25°C for 1 month before amplification, target capture and/or sequencing. To avoid degradation, always store DNA in a buffered solution (10 mM Tris-HCl, pH 8.0 – 8.5) when possible, and minimise the number of freeze-thaw cycles.
KAPA Adapters are recommended for use with the KAPA EvoPrep Kits. For assistance with third-party adapter compatibility and ordering, please contact Technical Support for guidelines on the formulation of user-supplied library amplification primers.
KAPA EvoPlus V2 Kits
The KAPA EvoPlus V2 Kits offer improved fragmentation performance, insensitivity to fragmentation inhibitors, better conversion efficiency, and reduced sequencing artefacts* through our streamlined and fully automatable workflow. Bringing a new and upgraded enzymatic fragmentation and library prep solution to the market will enable researchers to achieve higher confidence with increased sequencing efficiency.
The KAPA EvoPlus V2 Kits offer a complete library preparation solution when combined with KAPA Adapters and KAPA HyperPure Beads (sold separately) and a complete target enrichment solution when combined with KAPA HyperCap or KAPA HyperPETE workflows. The kits are compatible with the Illumina sequencing platform and have been qualified with automation methods.
These and the many other advanced features of the KAPA EvoPrep Kits were thoughtfully designed to set new standards in NGS sample prep workflows and jumpstart the journey to your next big research breakthrough.
Benefits of the KAPA EvoPlus V2 Kits*
* Compared to earlier KAPA DNA Libray Prep chemistries. Data on file with Roche.
- Broad compatibility and flexibility
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Compatible with a wide range of sample types, buffers and inputs, and flexible with respect to fragment size, adapter design, and library amplification
- Simplified and streamlined workflow
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Simplified, streamlined, and automatable workflow with ReadyMix reagents, available in tube and plated formats to increase efficiencies and convenience
- Enhanced sequencing performance with fewer artefacts
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Drastically reduced sequencing artefacts, with insensitivity to inhibitors, fully tunable fragmentation, and improved library prep performance
Achieve even greater confidence with KAPA EvoPlus V2's enhanced efficiency
Frequently asked questions about KAPA EvoPlus V2
- Whole-genome shotgun sequencing
- Whole-exome sequencing, using KAPA HyperCap Evolved workflow v4.x
- Targeting sequencing, using KAPA HyperCap or KAPA HyperPETE workflows
The library construction process, from enzymatic fragmentation to final library, can be performed in ~ 2 hours depending on experience, the number of samples being processed, and whether or not library amplification is performed. If necessary, the protocol may be paused safely after completion of the post-ligation cleanup or after post-amplification cleanup.
Purified, adapter-ligated library DNA may be stored at 2°C to 8°C for 1 – 2 weeks, or at -15°C to -25°C for 1 month before amplification, target capture and/or sequencing.
Conditioning solution not required for the KAPA EvoPlus V2 Workflow. KAPA FragTail Readymix is insensitive to inhibitors.
KAPA Adapters are recommended for use with the KAPA EvoPlus V2 Kits. For assistance with third- party adapter compatibility and ordering, please contact Technical Support for guidelines on the formulation of user-supplied library amplification primers.