KAPA Universal Adapter
The KAPA Universal Adapter is a short (truncated) adapter for next-generation sequencing (NGS) on Illumina platforms. It carries T-overhangs and ligates to the library fragments after the A-tailing step. Sample barcodes are incorporated during the following library amplification step prior to target enrichment.
The KAPA Unique Dual-Indexed (UDI) Primer Mixes are to be used with the KAPA Universal Adapter to generate uniquely labeled libraries from individual biological DNA samples.
Each KAPA UDI Primer Mix is a pre-mixed combination of forward and reverses primers.
This adapter has been manufactured by duplexing and is designed to ensure high library construction ligation efficiency and low adapter-dimer formation.
This Roche Sequencing product is part of the validated KAPA HyperCap workflow & KAPA HyperPETE Workflow, a streamlined, automation-friendly solution that integrates KAPA’s high-efficiency DNA library preparation kits with the KAPA Target Enrichment Probes (Exome and Custom).