The LightMix® Digital BRAF V600K assay kit is meticulously designed for the detection of the BRAF V600K mutation (c.1798_1799delGTinsAA) in human genomic DNA.
The kit includes air-dried oligo mixes with FAM/HEX-labeled probes and BHQ2 quenchers for accurate mutation analysis. The amplicon length is 81 bp, and a positive control with a 4-6% plasmid insert in genomic DNA is included to ensure reliable results.
This research-use-only kit provides sensitive detection of the BRAF V600K mutation in genomic DNA samples. The analytical sensitivity is verified to be less than 0.1%, making it ideal for mutation detection and genomic studies.
- Storage: Upon arrival, store the kit at 4°C to 25°C. The reconstituted reagents are stable for up to 60 days at 4°C to 8°C. For long-term storage, keep the reagents at -15°C to -25°C.
- Application: The LightMix® Digital BRAF V600K assay kit is perfect for research in oncology, mutation detection, and genomic analysis, providing high sensitivity and reliable results.
