The KAPA EvoPlus V2 Kits offer improved fragmentation performance, insensitivity to fragmentation inhibitors, better conversion efficiency, and reduced sequencing artefacts through our streamlined and fully automatable workflow. Bringing a new and upgraded enzymatic fragmentation and library prep solution to the market will enable researchers to achieve higher confidence with increased sequencing efficiency.
The KAPA EvoPlus V2 Kits offer a complete library preparation solution when combined with KAPA Adapters and KAPA HyperPure Beads (sold separately). The kits are compatible with the Illumina sequencing platform and have been qualified with automation methods.
Features and Benefits of KAPA EvoPlus V2 Kits
- Drastically reduced sequencing artefacts with insensitivity to inhibitors, fully tunable fragmentation and improved library prep performance
- Simplified, streamlined and automatable workflow, with combined Fragmentation and A-tailing step in ReadyMix formulations
- Tubes and plated format increases efficiency and convenience
- Compatible with a wide range of sample types and inputs, and flexibility with respect to fragment size, adapter design and library amplification
Product highlights
- Enable superior performance and sequencing results
- KAPA EvoPlus V2 Kits provide the benefits of enzymatic fragmentation without drawbacks
- Improved sequencing metrics, allowing higher confidence in data due to reduction in sequencing artefacts
Simplified and streamlined workflow
- Streamlined library prep with combined Fragmentation and A-tailing step
- ReadyMix formulations, therefore fewer reagents and hands-on-time
- Tubes and plated format, increased efficiency and convenience
- Manual and automation friendly
- Reduces complexity of workflow and provides greater peace of mind
- Validated with the KAPA HyperCap Workflow and KAPA HyperPETE Workflow
Figure 1: The KAPA EvoPlus V2 Workflow
The KAPA EvoPlus V2 Kit provides a simplified and streamlined workflow to remove the complexities and risk for human error by providing a trusted enzymatic fragmentation solution.
Tunable and trustable fragmentation
- Library insert sizes adjustable by varying fragmentation time
- Reproducible insert sizes across a range of GC content and DNA input amounts
- No impact to fragmentation – insensitive to EDTA (up to 2 mM), as well as numerous other inhibitors
See performance data showing tunability and consistency in DNA library insert sizes with samples from different input amounts and DNA dilution buffer.
Figure 2: The KAPA EvoPlus V2 chemistry enables tunable enzymatic fragmentation
Human genomic DNA (100 ng) was fragmented at 37°C for different periods of time (5 – 30 min) to achieve mode library insert sizes ranging from approximately 250 – 1000 bp. The KAPA EvoPlus V2 workflow was completed without any size selection using full-length adapters (KAPA UDI Adapters) and 3 cycles of amplification to enable visualisation. Electropherograms were generated with LabChip GX Touch NGS 3K Assay.
Learn more about the product visiting its eLabDoc File or find here the Operator’s manual.
