The KAPA RNA EvoPrep Kit with RiboErase (HMR or Human/Mouse/Rat) Globin contains all of the buffers and enzymes required for depletion of ribosomal RNA (rRNA) and globin mRNA transcripts, and the rapid construction of stranded RNA-seq libraries from 5 ng – 1 μg of blood-derived, purified total RNA.
The KAPA RNA EvoPrep Kit with RiboErase (HMR) Globin is designed for both manual and automated NGS library construction.
The kit depletes both cytoplasmic (5S, 5.8S, 18S, and 28S), and mitochondrial (12S and 16S) rRNA species as well as globin mRNA transcripts.
The protocol is applicable to a wide range of RNA-Seq applications, including:
- gene expression analysis;
- single nucleotide variation (SNV) discovery;
- splice junction and gene fusion identification; and
- characterization of both polyadenylated and non-polyadenylated RNAs, including noncoding and immature RNAs.
This kit has not been tested with small RNAs < 100 bp in length.
